Directions

  • Select all methylation files of each type when uploading data from the single-cell experiment. This summarized data should be from Bismark's methylation extractor or in the form of three columns (chromosome, position, methylation rate).
  • As these files are large, we process a single chromosome at a time. Indicate the desired chromosome as a number (e.g., 1 - 22) or as X, Y, MT.
  • After pressing 'Run', a progress bar will appear in the bottom right.

After processing and subsetting the data for visualization, you may download the data below. The data will be downloaded in RDS format and can be loaded into the Visualization tab.

Download Processed Data


For additional information or to view Frequently Asked Questions, see the methylscaper vignette.

Questions can also be submitted on our GitHub page: Report issue/bug

Upload the RDS file obtained in the Preprocessing tab below in the input labelled 'RDS File Input'. Alternatively, explore the app by selecting 'Load Example Data' below. The example data is from Mouse on Chromosome 19 subset to a 40 kbp region around 8,967,041. Some genes that are located in this region are Eef1g, Mta2, and Tut1.

To move along the genome, we have pre-loaded gene locations for Human (GRCh38) and Mouse (GRCm39) for the chromosome selected in the Preprocessing tab. Select a gene and then a slider will appear to refine the genomic location.

With Brushing set to Weighting, drag the mouse/cursor to select basepairs (columns) on which to weight the ordering algorithm. Once the weighting is set, change Brushing to refinement and highlight the cells (rows) to refine the ordering.

Detailed descriptions of the various options for seriation, weighting, and refinement are in the methylscaper vignette.

Download Heatmap Download Ordering Log

Below are summary plots of the methylation data. The histogram on the left calulates the proportion of methylated bases within each cell. Choose whether to calculate this for the endogenous methylation or the accessibility methylation. The plot in the middle calculates the percent of cells having methylation at each GCH (yellow) or HCG (red) site in the region. The plot on the right calculates a population averaged methylation status over the entire region. The window size can be adjusted. This plot is similar to the one in the middle but is easier to interpret when the region is large.



Directions

  • Upload the reads or sequences from the single-molecule experiment in FASTA format in the input labelled 'FASTA File'. There is a 10MB upload limit for files on the webserver. For larger files, please run methylscaper locally. Additional details are provided in the vignette linked at the bottom of this page.
  • Upload the reference sequence for the gene or genomic location of interest in the input labelled 'Reference File', this should also be in FASTA format.
  • After pressing 'Run', a progress bar will appear in the bottom right.

After aligining and processing the reads for visualization, you may download the data and the preprocessing log below. The data will be downloaded in RDS format and can be loaded into the Visualization tab. The processing log contains details on the read alignments.

Download Processed Data Download Log File


For additional information or to view Frequently Asked Questions, see the methylscaper vignette.

Questions can also be submitted on our GitHub page: Report issue/bug

Upload the RDS file obtained in the Preprocessing tab below in the input labelled 'RDS File Input'. Alternatively, explore the app by selecting 'Load Example Data'.

With Brushing set to Weighting, drag the mouse/cursor to select basepairs (columns) on which to weight the ordering algorithm. Once the weighting is set, change Brushing to refinement and highlight the molecules (rows) to refine the ordering.

Detailed descriptions of the various options for seriation, weighting, and refinement are in the methylscaper vignette.

Download Heatmap Download Ordering Log

Below are summary plots of the methylation data. The histogram on the left calulates the proportion of methylated bases within each molecule. Choose whether to calculate this for the endogenous methylation or the accessibility methylation. The plot in the middle calculates the percent of molecules having methylation at each GCH (yellow) or HCG (red) site in the region. The plot on the right calculates a population averaged methylation status over the entire region. The window size can be adjusted. This plot is similar to the one in the middle but is easier to interpret when the region is large.